Journal: Cell reports

Article Title: Principles of assembly and regulation of condensates of Polycomb repressive complex 1 through phase separation

doi: 10.1016/j.celrep.2023.113136

Figure Lengend Snippet: (A) A hypothetical model describing how condensate composition regulates the partitioning of CBX2-PRC1 components and nucleosomes and the exchange properties of the scaffold CBX2. Colored hexagons are the CBX2-PRC1 clients (magenta) and nucleosomes (green). (B) Representative epi-fluorescence images of CBX2-PRC1 subunits in the four-component (CBX2, RING1B [R], MEL18 [M], and PHC1 [P]) system. Scale bars, 5.0 μm. (C) Box plot of condensed fraction in the four-component system quantified from (B). p value is calculated using Student’s t test (*p < 0.05; **p < 0.01). (D) FRAP curves of CBX2 in the single-component, two-component, three-component, and four-component systems. Error bars denote SD. (E) Example confocal fluorescence images of CBX2 and nucleosomes (Nuc.) in the two-component, three-component, four-component, and five-component systems. Scale bars, 5.0 μm. (F) Box plot of condensed fraction of CBX2 and nucleosomes quantified from (E). p value is calculated using Student’s t test (**p < 0.01). (G) FRAP curves of YFP-CBX2 in the two-, three-, four-, and five-component systems. Error bars denote SD. (H) Representative live-cell epi-fluorescence images of HT-CBX2 in wild-type (WT), Ring1a −/− /b −/− , and Bmi1 −/− /Mel18 −/− mESC lines. Scale bars, 5.0 μm. (I and J) Box plots of condensed fraction (I) and size (J) of HT-CBX2 condensates quantified from (H). p value is calculated using Student’s t test (**p < 0.01). Error bars denote SD. (K) Example confocal images of FRAP of HT-CBX2 in wild-type (WT), Ring1a −/− /b −/− , and Bmi1 −/− /Mel18 −/− mESC lines. Red arrows show condensates to be bleached. Scale bar, 5.0 μm. (L) FRAP curves of HT-CBX2 within and outside condensates in wild-type (WT), Ring1a −/− /b −/− , and Bmi1 −/− /Mel18 −/− mESC lines. Error bars denote SD.

Article Snippet: The FRAP curves were fitted by a single-exponential function implemented in OriginLab as described in. (Equation 5) I = r − m ⋅ e ( − k ⋅ t ) Where m is the mobile fraction.

Techniques: Fluorescence

Journal: Nanomaterials

Article Title: The Mechanical Properties of Blended Fibrinogen:Polycaprolactone (PCL) Nanofibers

doi: 10.3390/nano13081359

Figure Lengend Snippet: Incremental stress–strain curves. ( A ) Strain versus time curve for electrospun 75:25 fibrinogen:PCL fiber. The fiber was pulled to a small strain (~10%) and held constant for approximately 30–40 s; this process was repeated with a slightly larger strain at each time. ( B ) Stress versus time curve. At constant strain, the stress relaxes and decays exponentially with time. ( C ) Representative stress relaxation curves. A double exponential curve is fitted to the relaxation curve (R 2 = 0.99) to determine the relaxation times. The fast and slow relaxation times for this curve were 1.8 s and 21 s. ( D ) Moduli versus strain curve. The total modulus, Y tot , (stars) and relaxed, elastic modulus, Y 0 , (dots) decrease as the strain increases. ( E ) The graph shows statistical differences between the slow and fast relaxation times of the fibers with two different ratios. The fiber diameter was 99 nm. ** indicates a p -value < 0.01; **** indicates a p -value < 0.0001.

Article Snippet: Individual stress relaxation curves were fitted to this double exponential function in Origin (OriginLab Corporation, Northampton, MA, USA).

Techniques: